Non-Canonical Amino Acids as Minimal Tags for Investigating Protein Organization and Turnover
Author | : Ingrid-Cristiana Gebura-Vreja |
Publisher | : |
Total Pages | : 161 |
Release | : 2015 |
ISBN-10 | : OCLC:934221355 |
ISBN-13 | : |
Rating | : 4/5 (55 Downloads) |
Download or read book Non-Canonical Amino Acids as Minimal Tags for Investigating Protein Organization and Turnover written by Ingrid-Cristiana Gebura-Vreja and published by . This book was released on 2015 with total page 161 pages. Available in PDF, EPUB and Kindle. Book excerpt: The burgeoning field of genetic code expansion provides new tools for specifically labeling proteins for a variety of applications. Clickable non-canonical amino acids (ncAA) have been refined for almost-quantitative and highly selective reactions with complementary probes. In my thesis work I have adapted genetic code expansion for two biological questions. First, I used ncAA incorporation and click reaction to test the effect of fluorescent protein (FP) tagging on the nanoscale organization of target proteins. Second, I used these tools to generate a genetically-encoded scheme for specifi...