Download or read book Delineation of Structural Domains of the Sodium/hydrogen Exchanger Isoform 1 written by Pietro Iannuzzi and published by . This book was released on 2000 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The last objective was to define the membrane topology of NHE1. The methodology involves reintroducing cysteine residues into a cysteine-less mutant NHE1 and assessing their accessibility with thiol-reactive agents. Unfortunately, these results were inconclusive and further optimization of the assay conditions is required." --